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http://nopr.niscpr.res.in/handle/123456789/30470| Title: | Screening of apple germplasm of Kashmir Himalayas for scab resistance genes |
| Authors: | Khajuria, Yash Paul Kaul, Sanjana Wafai, B A Dhar, Manoj K |
| Keywords: | Apple scab;Disease resistance gene;Malus × domestica;Venturia inaequalis;Vf gene |
| Issue Date: | Oct-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In India, apple (Malus × domestica) is grown on a large scale in Kashmir province (valley) of the Jammu and Kashmir State. We surveyed several orchards of the valley in order to assess the germplasm. Apple scab was found to be the one of most important diseases, which is caused by the fungal pathogen, Venturia inaequalis. For the present investigation, 80 cultivars were screened for the presence of known genes conferring disease resistance. Different cultivars of apple showed variable response to apple scab. Of the four resistant cultivars identified previously, namely two, Shireen and Firdous were found to harbour Vf (Rvi6) gene as the source of resistance. Other two resistant cultivars were found to lack this gene. Therefore, it appears that these cultivars may have either other known or novel scab resistance gene(s). Using specific primers, PCR technique was used to amplify Vf (Rvi6) specific fragments, which were later cloned, sequenced and further characterized by using bioinformatics tools. These clones showed similarity with different clones of Vf gene isolated from M. floribunda by earlier workers. Bioinformatics analysis showed some similarity of cloned sequences with serine-threonine kinases that help the plant to overcome various stresses. However, further work is needed to confirm this observation. |
| Page(s): | 448-454 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(4) [October 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(4) 448-454.pdf | 382.65 kB | Adobe PDF | View/Open |
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