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http://nopr.niscpr.res.in/handle/123456789/30471| Title: | Identification of simple sequence repeat markers tightly linked to lipoxygenase-2 gene in soybean |
| Authors: | Kumar, Vineet Rani, Anita Rawal, Reena |
| Keywords: | Lipoxygenase-2;Lox2 locus;Off-flavour;Soybean;SSR markers |
| Issue Date: | Oct-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Lipoxygenase-2, controlled by single dominant gene, is prime cause of off-flavour generated in soy products. The objective of the present investigation was to validate the SSR markers (Sat_074 & Satt522) reported to be linked to Lox2 locus and to identify tightly linked SSR markers in F2 mapping population 1 and population 2, generated from crosses between Indian soybean varieties JS97-52 and JS93-05 as the conventional parents (Lox2Lox2) and PI596540 as the source of null allele of lipoxygenase-2 (lox2lox2), respectively. Parental polymorphism was surveyed using SSR markers Sat_074, Satt522 and 7 SSR markers in the neighbouring genomic region of Lox2 locus. F2:3 seeds from each F2 individual in both the mapping populations were phenotyped for the presence/absence of lipoxygenase-2 isozyme. The SSR marker Sat_074 was not found to be polymorphic for the parental combination JS97-52 x PI596540. Satt522 was found to be at 9.3 and 13.5 cM from Lox2 locus in mapping population 1 and 2, respectively. We found two additional new SSR markers Sat_417 and Satt656 closely linked to Lox2 locus. SSR marker Sat_417 was found to be at a close distance of 6.9 and 6.6 cM from Lox2 locus; while Satt656 was identified to be tightly linked to Lox2 locus at a distance of 2.7 and 2.1 cM in the mapping population 1 and 2, respectively. Thus, the SSR marker Satt656 can be employed in marker assisted selection for transferring null allele (lox2) of the lipoxygenase-2 in the background of popular Indian soybean cultivars. |
| Page(s): | 455-458 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(4) [October 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(4) 455-458.pdf | 107.22 kB | Adobe PDF | View/Open |
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