<span style="mso-ansi-language:EN-US" lang="EN-US">Effect of different factors on <i>in vitro </i>axillary shoot proliferation and plant regeneration of <i>Cinnamomum tamala </i>Nees: A spice yielding plant </span>

Abstract

<span style="mso-ansi-language:EN-US" lang="EN-US">In the present study, a successful attempt was made to establish the aseptic<i> </i>culture of <i>Cinnamomum tamala</i> Nees from nodal explants and to evaluate the factors influencing <i>in vitro</i> morphogenesis. About 45.6% nodal explants collected during July established successfully on full strength MS medium fortified with sucrose (3%, w/v), polyvinyl pyrollidone (PVP; 100 mg L^-1) and benzyl adenine (BA; 9 µM), where as many as 5.4 meristematic loci were invoked after 5-6 wk of culture initiation. The shoot buds developed on initiation medium converted into microshoots on transferring to MS medium conjunct with sucrose (3%) and kinetin (Kn; 3 µM). The microshoots were rooted by culture on MS medium fortified with sucrose (3%) and α-naphthalene acetic acid (NAA; 3 µM), where as many as 10 roots developed per microshoot. The rooted plantlets were transferred to potting mix containing soil, sand and decayed wood powder at 1:1:1 ratio and maintained in the polyhouse at <i>ca</i> 75% shading of light for 2 months. The acclimatized plantlets were transferred to the nature and registered 70% survival after 2 months of transfer.

</span>