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http://nopr.niscpr.res.in/handle/123456789/30481| Title: | Direct organogenesis in some soybean genotypes using cotyledonary segments |
| Authors: | Hai, Huynh Ngoc Lal, Sanjay Kumar Singh, Sanjay Kumar Prabhu, Kumble Vinod Talukdar, Akshay Sapra, Ramesh Lal |
| Keywords: | Glycine max;in vitro plant regeneration;Mature cotyledon segment |
| Issue Date: | Oct-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Effect of various parameters on direct shoot organogenesis and plantlet regeneration from mature cotyledonary segments of three soybean [Glycine max (L.) Merrill] genotypes, viz., JS 335, Pusa 9814 and Pusa 24, was studied. The seeds of these genotypes were germinated in vitro on Murashige and Skoog medium supplemented with 9.0 μM BAP (6-benzylamino purine)+1.1 μM NAA (α-naphthalene acetic acid). After 10 d, green cotyledonary segments were excised from the germinating seedlings and used as explant (0.25-0.4 cm). These segments were cultured on B5 medium supplemented with 6.8 μM BAP+0.73 μM GA3 (gibberellic acid)+0-20 mM MES (2-morpholino ethane sulphonic acid). Genotype Pusa 24 was found most responsive (32.23%) for direct shoot organogenesis, followed by Pusa 9814 (22.57%) and JS 335 (7.93%). The regenerated microshoots were elongated on MS medium supplemented with 2.9 μM GA3. Medium comprising MS basal salts supplemented with 5.0 μM IBA (indole-3-butyric acid) and 5.4 μM NAA was found ideal for root induction on microshoots. Rooted plants were successfully hardened in glass jars with polypropylene cap and established in the greenhouse with 87% survival rate. |
| Page(s): | 527-531 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(4) [October 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(4) 527-531.pdf | 141.57 kB | Adobe PDF | View/Open |
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