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http://nopr.niscpr.res.in/handle/123456789/30483| Title: | A simple and rapid DNA extraction protocol for filamentous fungi efficient for molecular studies |
| Authors: | Gontia-Mishra, Iti Tripathi, Niraj Tiwari, Sharad |
| Keywords: | DNA isolation;Filamentous fungi;PCR-RFLP;RAPD;18S rRNA |
| Issue Date: | Oct-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A simple and rapid protocol for extracting high-quality DNA from filamentous fungi was studied. The method involved disruption of fungal cells by employing glass bead method, followed by inactivation of proteins using CTAB/proteinase K. The DNA yield from fungal isolates varied from 310-1879 µg g-1 dry mycelium and a clear intact DNA band was observed upon agarose gel electrophoresis. Absorbency ratios (A260/A280) for DNA ranged 1.7-1.9, which indicated minimal presence of contaminating metabolites. PCR analysis like 18S rRNA gene amplification, random amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) showed that DNA was compatible for downstream applications. This method can be applied to extract genomic DNA of filamentous fungi from different environmental sources. |
| Page(s): | 536-539 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.13(4) [October 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 13(4) 536-539.pdf | 56.86 kB | Adobe PDF | View/Open |
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