Comparative study of cytokinin production isolated from bacteria and shoot induction

Abstract

<i style="mso-bidi-font-style:normal">Rhizobium</i> sp., isolated from <i style="mso-bidi-font-style: normal">Mimosa pudica</i>, <i style="mso-bidi-font-style:normal">Bacillus</i> <i style="mso-bidi-font-style:normal">subtilis</i> BC1 and <i style="mso-bidi-font-style: normal">Escherichia coli </i>K12 were investigated for their ability to produce plant growth hormones in a given production media (PM1 & PM2). Thin layer chromatography and high pressure liquid chromatography were performed to analyse the amount of cytokinin produced by these microorganisms. <i style="mso-bidi-font-style:normal">E. coli </i>K12 produced the highest amount (0.186 mg/L) of cytokinin in PM2 medium, while <i style="mso-bidi-font-style: normal">B. subtilis</i> BC1 gave the least production amount (0.0075 mg/L) in the same medium. The microbially synthesized cytokinin was then incorporated onto the solidified MS medium for the shoot development in <i style="mso-bidi-font-style:normal">Phillodendron xanadu</i>, an ornamental plant. The<span style="mso-bidi-font-style:italic"> cytokinin<i> </i>produced from <i>E. coli </i><span style="mso-bidi-font-style:italic">K12 gave more number of plantlets with thick shoot and lustrous leaves having 3.0 cm length for medium with charcoal (PM2); while comparatively less number of plantlets having 1.5 cm length for medium without charcoal. The cytokinin from<i style="mso-bidi-font-style:normal"> B.</i> <i style="mso-bidi-font-style:normal">subtilis</i> BC1<span style="mso-bidi-font-style:italic"> and <i>Rhizobium </i>sp. gave comparatively more shoot number and more plant height on medium with charcoal in PM1 and PM2 respectively.

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