Genetic diversity in tetraploid and diploid cotton (Gossypium spp.) using ISSR and microsatellite DNA markers

Abstract

Two marker systems viz., microsatellite and ISSR were used to generate DNA profiles of 19 genotypes of cotton (diploid and tetraploid), and each major DNA fragment was recorded. The results of cluster analysis indicated not only the separation of genotypes of the two major species (Gossypium arboreum and G. hirsutum) into separate groups, but also the separation of the genotypes among each group. The similarity coefficient based on ISSR and microsatellite markers ranged from 0.59 to 0.90 and 0.59 to 0.93, respectively, thus suggesting considerable genetic variation between the cotton species studied.