Lipophilic metabolite profiling of maize and sorghum seeds and seedlings, and their pest spotted stem borer larvae: A standardized GC-MS based approach

Abstract

In order to better understand the biochemical interactions and to identify new biomarkers for plant resistance against insects, we proposed a suitable lipophilic profiling method for insects and their host plants. The critical components of GC-MS based analysis are: sample amount, extraction, derivatization, temperature gradient, run time, and identification of peaks. For lipophilic metabolite profiling of maize and sorghum, and their insect pest, <span style="mso-bidi-font-weight:bold">spotted stem borer larvae, we recommend 100 mg sample weight for seeds and insect samples (whole insect body), and 200 mg for seedlings. <span style="mso-bidi-font-weight:bold">Maize and sorghum seeds required less time for fat extraction in comparison to their seedlings and the pest<span style="mso-bidi-font-weight:bold"> fed on these seedlings. GC-MS was standardized for better separation and intensity of peaks using different temperature gradients in the range of 180-300 C. A total of 48 lipophilic compounds encompassing various classes based on their functional groups such as fatty acids, fatty alcohols, hydrocarbons, sterols and terpenoids, vitamin derivative, etc. were separated in the seedlings (30), seeds (14), and the pest (26) in the retention time range of 3.22 to 29.41 min. <span style="mso-bidi-font-weight:bold">This method could be useful to study nutritional aspects of different field crops in relation to various stresses<span style="mso-bidi-font-weight:bold"> apart from the analysis of lipophilic compounds for better understanding of insect-plant interactions.

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