Enzymatic characteristics of lignin peroxidases of indigenous lignolytic fungal strains — Part I

Abstract

Extracellular production of lignin peroxidases from six indigenous fungi, viz. Abortiporus biennis MTCC-1176, Pestalotia bicolor MTCC-372, Heterobasidium annosum MTCC-146, Pleurotus ostreatus MTCC-142, Gloephyllum striatum MTCC-1117 and Loweporus lividus MTCC-1178, in liquid culture growth medium amended with lignin-containing natural substances and their enzymatic characteristics were studied. P. ostreatus MTCC-142 was found to be the best secretor of lignin peroxidase (3.50 IU/mL) in the presence of saw dust. Moreover, the enzymatic characteristics (Km, pH and temperature optima) of the lignin peroxidases produced by indigenous fungal strains were in the same range as the enzymatic characteristics of Phanerochacte chrysosporium ATCC-24725, an American type culture collection widely used for purification of lignin peroxidase. Thus, P. ostreatus MTCC-142 has potential to be used for commercial production of lignin peroxidase