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http://nopr.niscpr.res.in/handle/123456789/30879| Title: | Comparison of catalytic activity of immobilized amyloglucosidase on DEAE-cellulose with native enzyme on dextrin-hydrolysis |
| Authors: | Rani, AS Das, M L M Rao, A Rajeshwar Satyanarayana, S |
| Issue Date: | Nov-1998 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Amyloglucosidaseis an exo-enzyme which is used in the saccharification of starch in industries. In this study the catalytic activity of amyloglucosidase immobilized on DEAE-cellulose (diethyl amino ethylcellulose) was investigated and compared with native enzyme on hydrolysis of dextrin. Amyloglucosidase was immobilized on DEAE-cellulose by ionic binding method. Activity of DEAE-cellulose immobilized and native enzymes were compared and it was observed that mobilized amyloglucosidase has 78% of the catalytic activity of native enzyme. The activity of DEAE-cellulose immobilized enzyme as a function of pH and temperature was determined. Kineti cconstants Km and Vmax were also determined. |
| Page(s): | 397-401 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Appears in Collections: | IJCT Vol.05(6) [November 1998] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCT 5(6) 397-401.pdf | 365.08 kB | Adobe PDF | View/Open |
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