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http://nopr.niscpr.res.in/handle/123456789/30880Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Pandey, P C | - |
| dc.date.accessioned | 2015-03-12T09:22:21Z | - |
| dc.date.available | 2015-03-12T09:22:21Z | - |
| dc.date.issued | 1998-11 | - |
| dc.identifier.issn | 0975-0991 (Online); 0971-457X (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/30880 | - |
| dc.description | 402-404 | en_US |
| dc.description.abstract | An optical sensor for nitric oxide (NO) is described. The sensor involves a flow injection analysis (FIA) system coupled with an evanescent wave (EW) sensor employing total internal reflection of fluorescence radiation (TIRF). The detection is based on the measurement of the decrease in fluorescence response in the presence of NO of a highly fluorescent product which is the complex of glutathione and 7-diethylamino-3-(4'-maleimidylphenyl)-4methylcoumarin (CPM). CPM forms a highly fluorescent product with thiol containing compound i.e. cysteine/glutathione which can be detected using an evanescent fluorosensor. NO form a stable complex with glutathione/cysteine which then does not react with CPM to form fluorescent product. Thus there is a decrease in the fluorescence signal on increasing concentration of NO with constant concentration of glutathione. An excellent FIA response has been observed on the injection of the constant concentration of CPM-glutathione product obtained by treating constant concentration of glutathione with varying concentration of NO. The lowest detection limit of NO is found to the order of 0.10 µM. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | IJCT Vol.05(6) [November 1998] | en_US |
| dc.title | Detection of nitric oxide using an optical sensor | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJCT Vol.05(6) [November 1998] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCT 5(6) 402-404.pdf | 214.93 kB | Adobe PDF | View/Open |
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