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http://nopr.niscpr.res.in/handle/123456789/31234| Title: | Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf. |
| Authors: | Bouaziz-Ketata, Hanen Zouari, Nabil Salah, Hichem Ben Rafrafi, Moez Zeghal, Najiba |
| Keywords: | Apigenin;Catechin;Diosmetin;HPLC;LC-ESI-MS;Luteolin;Methylquercetin;Tangeritin;Thatching grass |
| Issue Date: | Apr-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In this study, we report isolation of flavonoids, viz., 3-O-methylquercetin, tangeritin, luteolin-7-O-glucoside, luteolin, apigenin-7-O-glucoside, apigenin-8-C-glucoside, luteolin-8-C-glucoside, luteolin-6-C-glucoside, diosmetin and catechin from the methanolic extract of Hyparrhenia hirta employing high performance liquid chromatography and liquid chromatography-electrospray ionization-tandem mass spectrometry. The total phenolic content of H. hirta extract was 105.58 ± 0.1 mg gallic acid equivalents/g of plant extract while the total flavonoid content was 45.20 ± 0.2 mg quercetin equivalents/g of plant extract and the total condensed tannin were 72.35 ± 0.7 mg catechin equivalents/g of plant extract by reference to standard curve. The antioxidant activity was assayed through the antioxidant capacity by phosphomolybdenum assay, the reducing power assay and the radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method. The extract showed dose dependant activity in all the three assays. |
| Page(s): | 208-215 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Appears in Collections: | IJEB Vol.53(04) [April 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 53(4) 208-215.pdf | 231.8 kB | Adobe PDF | View/Open |
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