Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/31259
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dc.contributor.authorChakraborti, Tapati-
dc.contributor.authorDas, Partha-
dc.contributor.authorChoudhury, Rajdeep-
dc.contributor.authorDe, Tripti-
dc.date.accessioned2015-03-30T04:39:05Z-
dc.date.available2015-03-30T04:39:05Z-
dc.date.issued2015-02-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/31259-
dc.description14-22en_US
dc.description.abstractProteases have been considered as an important group of targets for development of antiprotozoal drugs due to their essential roles in host-parasite interactions, parasite immune evasion, life cycle transition and pathogenesis of parasitic diseases. The development of potent and selective serine protease inhibitors targeting L. donovani secretory serine protease (pSP) could pave the way to the discovery of potential antileishmanial drugs. Here, we employed different classical serine protease inhibitors (SPIs), such as aprotinin, N-tosyl-l-phenylalanine chloromethyl ketone (TPCK), N-tosyl-lysine chloromethyl ketone (TLCK), benzamidine (Bza) and pSP-antibody to determine the role of the protease in parasitic survival, growth and infectivity. Among the different classical SPIs, aprotinin appeared to be more potent in arresting L. donovani promastigotes growth with significant morphological alterations. Furthermore, aprotinin and anti-pSP treated parasites significantly decreased the intracellular parasites and percentage of infected macrophages. These results suggest that SPIs may reduce the infectivity by targeting the serine protease activity and may prove useful to elucidate defined molecular mechanisms of pSP, as well as for the development of novel antileishmanial drugs in future.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.52(1) [February 2015]en_US
dc.subjectLeishmania donovanien_US
dc.subjectSerine proteaseen_US
dc.subjectSerine protease inhibitorsen_US
dc.subjectAnti-leishmanial activityen_US
dc.titleEffect of different serine protease inhibitors in validating the 115 kDa Leishmania donovani secretory serine protease as chemotherapeutic targeten_US
dc.typeArticleen_US
Appears in Collections:IJBB Vol.52(1) [February 2015]

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