Induced defence responses of contrasting bread wheat genotypes under differential salt stress imposition

Abstract

Plants, being sessile in nature, have developed mechanisms to cope with high salt concentrations in the soil. In this study, the effects of NaCl (50-200 mM) on expression of high-affinity potassium transporters (<i>HKTs</i>), antioxidant enzymes and their isozyme profiles were investigated in two contrasting bread wheat (<i style="mso-bidi-font-style:normal">Triticum aestivum </i>L.) genotypes viz., HD2329 (salt-sensitive) and Kharchia65 (salt-tolerant). Kharchia65 can successfully grow in salt affected soils, while HD2329 cannot tolerate salt stress. Differential expression studies of two <i style="mso-bidi-font-style:normal">HKT</i> genes (<i>Ta</i><i style="mso-bidi-font-style: normal">HKT2;1.1</i> and <i>Ta</i><i style="mso-bidi-font-style:normal">HKT2;3.1</i>) revealed their up-regulated expression (~1.5-fold) in the salt-sensitive HD2329 and down-regulated (~5-fold) inducible expression in the salt-tolerant genotype (Kharchia65). Specific activity of antioxidant enzymes, viz. superoxide dismutase (SOD), peroxidase (POX), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR) was found to be higher in the salt-tolerant genotype. Isozyme profile of two (POX and GR) antioxidant enzymes showed polymorphism between salt-tolerant and salt-sensitive genotypes. A new gene <i>Ta</i><i style="mso-bidi-font-style: normal">HKT2;3.1 </i>was also identified and its expression profile and role in salt stress tolerance in wheat was also studied<span style="letter-spacing: -.1pt;mso-ansi-language:EN-IN;mso-fareast-language:EN-IN">. Partial sequences of the <i>Ta</i><i style="mso-bidi-font-style:normal">HKT2;1.1</i> and <i>Ta</i><i style="mso-bidi-font-style:normal">HKT2;3.1</i> genes from bread wheat were submitted to the EMBL GenBank database. Our findings indicated that defence responses to salt stress were induced differentially in contrasting bread wheat genotypes which provide evidences for functional correlation between salt stress tolerance and differential biochemical and molecular expression patterns in bread wheat.

</span>