Production of 4-Ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of <i style="mso-bidi-font-style: normal">Ipomoea batatas</i> Lam.: A Comparative study

Abstract

A comparative study was done on the production of 4-ipomeanol from root tubers of <i style="mso-bidi-font-style: normal">Ipomoea batatas</i><span style="mso-bidi-font-style:italic"> and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 µM NAA (α-Naphthaleneacetic acid) and 1 µM KIN (Kinetin). Effect of various elicitors (<i>Fusarium solani</i>, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g^-1) amount of 4-ipomeanol. This is the first report on <i>in vitro</i> production of 4-ipomeanol from <i>I.</i><i> batatas</i>. Since 4-ipomeanol is reported to be present only in <i>I.</i><i> batatas</i>, this study would help in standardizing protocols for large scale production without affecting its natural flora.

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