Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/31485| Title: | Development of duplex RT-PCR for detection of Konjac mosaic virus and Spathiphyllum chlorotic vein banding virus in taro and peace lily |
| Authors: | Manikonda, Padmavathi Puthankalam, Srinivas Kalanghad Masarapu, Hema Pothur, Sreenivasulu |
| Keywords: | Duplex-RT-PCR;Konjac mosaic virus;Spathiphyllum chlorotic vein banding virus;Peace lily;Taro |
| Issue Date: | Jan-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Konjac mosaic virus (KoMV) and Spathiphyllum chlorotic vein banding virus (SCVbV) infecting aroids in Andhra Pradesh, India have recently been reported as distinct potyviruses. Primers were designed based on their partial genome sequence (GenBank A/C EU979524, GQ421462) to detect above viruses by duplex-reverse transcription-polymerase chain reaction (duplex RT-PCR). Using this method, the two viruses were individually detected in limited number of filed/nursery collected Colocasia esculenta (taro) and Spathiphyllum spp. (peace lily). No co-infections with two viruses were traced among the screened samples. However, they were simultaneously detected in the simulated leaf samples of taro and peace lily (1:1, w/w). |
| Page(s): | 120-122 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.14(1) [January 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 14(1) 120-122.pdf | 251.46 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.