<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:simsun;mso-bidi-font-family:mangal;="" mso-ansi-language:en-gb;mso-fareast-language:zh-cn;mso-bidi-language:hi"="" lang="EN-GB">Isolation, Screening and Optimization of Xylanase Production in Submerged Fermentation Using <i>P.citrinum</i>.</span>

Abstract

Cellulase free xylanase is one of the most important upcoming enzyme having industrial application in chemical, biochemical, pharmaceutical and food industry. In the present study, a soil isolate was found to be promising for xylanase production. Biochemical identification tests, Scanning Electron Microscopy (SEM) and 16S rRNA sequencing identified the organism <i>Penicillium citrinum</i> MTCC 9620. Later on, optimization of media composition and environmental factors were investigated. Temperature, pH, agitation speed, carbon sources, nitrogen sources, inoculum concentration, and additives were optimized on the basis of xylanase yield, dry cell weight, extracellular protein content, sugar consumption etc. Optimized media produced 138.73 U/mL xylanase at 120 h (30°C). The purified xylanase was found to have molecular weight approximately 29 KDa.