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http://nopr.niscpr.res.in/handle/123456789/31820| Title: | Optimization of inoculum density in hairy root culture of Plumbago rosea L. for enhanced growth and plumbagin production towards scaling-up in bioreactor |
| Authors: | Pillai, Dhanya B Jose, Binoy Satheeshkumar, K Krishnan, P N |
| Keywords: | Growth index;Inoculum density (ID);Plumbagin;Plumbago rosea L. |
| Issue Date: | Apr-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present study evaluated the influence of initial inoculum density (ID) on growth and plumbagin production in hairy root culture of Plumbago rosea L. in shake-flask as well as air-sparged bioreactor. IDs ranging from that of single root tip (0.08 g/L) to 10 g/L were analyzed in 50 mL MS basal media of 250 mL Erlenmeyer flasks. For scaling-up in bioreactor of 2 L working-capacity, 0.5, 1, 5, 10 and 15 g/L IDs were analysed. Optimal ID was found to be 1 g/L in shake-flask culture, producing a growth index of 36.15 on 30th d with plumbagin content of 1.325 g in 100 g DW of roots (1.325%). In the bioreactor, biomass increase was maximum for 5 g/L with a growth index of 11.428 and plumbagin content of 1.425%. Here we report the maximum plumbagin concentration (1.325%) in P. rosea hairy root culture, enhanced from 0.99% innate plumbagin content of the mother culture through sole optimization of ID. Further enhancement to 1.425% was attained by scaling-up in the bioreactor. The plumbagin concentration obtained in bioreactor culture of P. rosea hairy roots (1.425%) is also the highest reported so far in scaling-up. Thus results indicate that ID is a critical parameter influencing enhanced production of root biomass and plumbagin in hairy root cultures of P. rosea. |
| Page(s): | 264-269 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.14(2) [April 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 14(2) 264-269.pdf | 216.43 kB | Adobe PDF | View/Open |
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