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http://nopr.niscpr.res.in/handle/123456789/31821| Title: | Enzymatic transformation of the major ginsenoside Rb1 to compound K by Weissella hellenica DC06 |
| Authors: | Huq, Md Amdadul Kim, Yeon-Ju Min, Jin-Woo Siraj, Fayeza Md Siddiqi, Muhammad Zubair Yang, Deok-Chun |
| Keywords: | Compound K;Enzymatic transformation;Ginsenoside Rb1;Weissella hellenica |
| Issue Date: | Apr-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present study, the major ginsenoside Rb1 was transformed into the more pharmacologically active minor ginsenoside compound K (C-K) via the enzymatic activity of food grade Weissella hellenica DC06 isolated from kimchi, a traditional Korean fermented food. The transformation products were analyzed by TLC, HPLC, and LC/MS. Within 4 d of incubation, 100% of Rb1 was decomposed and converted into F2 and C-K. The crude enzyme of W. hellenica DC06 hydrolyzed the two glucose moieties attached to the C-3 position and the outer glucose moiety attached to the C-20 position of the ginsenoside Rb1. Strain DC06 hydrolyzed ginsenoside Rb1 along the following pathway: Rb1→Rd→F2→CK. The optimum temperature and pH of the enzyme activity was 37°C and 7.0, respectively. DC06 was identified as a W. hellenica species based on its 16S rRNA gene sequences through the construction of a phylogenetic tree and homology analysis. This is the first report of the bioconversion of ginsenoside Rb1 to ginsenoside C-K using W. hellenica DC06. |
| Page(s): | 270-275 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.14(2) [April 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 14(2) 270-275.pdf | 235.79 kB | Adobe PDF | View/Open |
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