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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Zhang, Fenxi | - |
| dc.contributor.author | Ren, Tongming | - |
| dc.contributor.author | Wu, Junfang | - |
| dc.contributor.author | Niu, Jie | - |
| dc.date.accessioned | 2015-08-05T11:21:42Z | - |
| dc.date.available | 2015-08-05T11:21:42Z | - |
| dc.date.issued | 2015-08 | - |
| dc.identifier.issn | 0975-1009 (Online); 0019-5189 (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/31909 | - |
| dc.description | 508-513 | en_US |
| dc.description.abstract | Bone marrow-derived mesenchymal stem cells (BMSCs) are the most promising seed cells for cell transplant. The proliferation of BMSCs is one of the most important determinants of the efficiency of MSC-based transplant therapy. It has been reported that transforming growth factor-β1 (TGF-β1) activates Wnt/β-catenin signaling and regulates cell proliferation. In this study, we investigated the effect of low concentrations of TGF-β1 on proliferation of BMSCs and the related mechanisms. BMSCs were treated with 0, 1, 5 and 10 ng/L recombinant mouse TGF-β1 for 12 h. Cell proliferation was measured by cell counting and MTT assay, and the proliferation-related signals p53, Mdm2, Akt1, Wnt3, phospho-Akt and β-catenin were measured by quantitative polymerase chain reaction (qPCR) and/or Western blot. Our results showed that TGF-β1 at low concentrations induced BMSC proliferation and expression of Mdm2, Akt1, phospho-Akt, Wnt3 and β-catenin, and inhibited p53 expression in dose dependent manner. Importantly, β-catenin siRNA significantly inhibited TGF-β1-induced BMSC proliferation. These findings suggest that low concentrations of TGF-β1 can stimulate proliferation of BMSCs, which is at least partially dependent on the activation of Wnt/β-catenin pathway. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | IJEB Vol.53(08) [August 2015] | en_US |
| dc.subject | BMSC proliferation | en_US |
| dc.subject | p53 expression | en_US |
| dc.subject | Stem cells | en_US |
| dc.subject | Transforming growth factor-β1 | en_US |
| dc.title | Small concentrations of TGF-β1 promote proliferation of bone marrow-derived mesenchymal stem cells via activation of Wnt/β-catenin pathway | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJEB Vol.53(08) [August 2015] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 53(8) 508-513.pdf | 274.1 kB | Adobe PDF | View/Open |
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