Optimization of extraction process of flavonoids in <i style="mso-bidi-font-style:normal">Phyllanthus emblica</i> L. by response surface methodology and content determination

Abstract

This study is done to optimize the extraction process of flavonoids in <i style="mso-bidi-font-style:normal">Phyllanthus emblica</i> L., and determine the quercetin content in the extract. Flavonoids in<i style="mso-bidi-font-style:normal"> Phyllanthus emblica</i> L. is extracted by solvent extraction, then, on the basis of single-fact or experiments, the process for extraction of flavonoids in <i style="mso-bidi-font-style: normal">Phyllanthus emblica</i> L. is optimized by response surface design with total flavonoid yield as the response. HPLC is used, with a Kromasil ODS-1 column, a mobile phase of acetonitrile-1 gm/L phosphoric acid solution (30:70) and a detection wavelength of 360 nm. The optimal process parameters for extraction of flavonoids in <i style="mso-bidi-font-style:normal">Phyllanthus emblica</i> L. obtained by response surface methodology are as follows: ethanol concentration of 60.14%, extraction temperature of 80<span style="mso-bidi-font-family:" times="" new="" roman""="" lang="EN-GB">°<span style="mso-hansi-font-family:SimSun" lang="EN-GB">C, liquid solid ratio of 25: 1 ml/gm and extraction time of 126.87 min. The process conditions could achieve a total flavonoid yield of 8.60%. Quercetin had a good linear relationship within 1.32~23.1 µg (r=0.9998); and average sample recovery was 101.90%, with a RSD of 2.10%. The response surface methodology had practical application value for the optimization of extraction of flavonoids, which can provide the production basis for industrial utilization of abundant <i style="mso-bidi-font-style: normal">Phyllanthus emblica</i> L. resources for total flavonoids extraction. The content determination method established can provide the foundation for the quality control of <i style="mso-bidi-font-style:normal">Phyllanthus emblica</i> L. extract.

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