<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Evaluation of food-drug interaction potential of <i>Aegle marmelos </i><span style="mso-bidi-font-style:italic">(L.) Corrêa through metabolism mediated cytochrome P450 inhibition assay</span></span>

Abstract

In the present study, possible cytochrome enzyme inhibition potential of <i>Aegle marmelos </i><span style="mso-bidi-font-style: italic">(L.) Corrêa fruit and its bioactive compound was evaluated. Interaction potential of <i>Aegle marmelos</i> extract was evaluated through CYP450-carbonmonoxide complex (CYP450-CO) assay. Additionally, influence on individual major human cytochrome P450 drug metabolizing enzymes such as CYP3A4, CYP2D6, CYP2C9 and CYP1A2 were studied through fluorescence screening assay. Bioactive compound was quantified through RP-HPLC, in order to standardize the sample material. <i>Aegle marmelos </i>extract showed higher IC₅₀ value than the respective standard inhibitors and lower than the bioactive compound imperatorin. <i>Aegle marmelos</i> extract showed significantly lesser (P<0.001, P<0.01) interaction potential than the standard inhibitors. The research findings indicated that selected sample is unlikely to cause clinically relevant drug interactions involving inhibition of major CYP isozymes.

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