<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Possible herb-drug interaction of <i style="mso-bidi-font-style:normal">Morus alba </i><span style="mso-bidi-font-style:italic">L<i style="mso-bidi-font-style: normal">.- a </i>potential anti-diabetic plant from Indian Traditional medicine</span></span>

Abstract

In the Indian System of Medicine, <i style="mso-bidi-font-style:normal">M. alba</i> L. leaves are widely used for the management diabetes and as a general tonic to enhance health. The present study was designed to standardize the extract of <i style="mso-bidi-font-style: normal">Morus alba</i> by reverse phase high performance liquid chromatography (RP-HPLC) and exploration of the herb drug interaction potential through inhibition of Cytochrome P450 (CYP450) enzymes. Quantification of bioactive constituent in the plant extract was determined by RP-HPLC method. The plant extract<i style="mso-bidi-font-style:normal"> </i>and single bioactive molecule were studied for their inhibition potential on pooled CYP450, as well as recombinant human CYP450 is forms such as CYP3A4, CYP2D6, CYP2C9 and CYP1A2 isozymes. The inhibition potential of the plant<i style="mso-bidi-font-style: normal"> </i>extract is higher than the single bioactive molecule. Plant extract and its phyto-constituent showed significantly higher IC₅₀ value than respective positive control against CYP3A4, 2D6, 2C9 and 1A2. Interaction potential of phyto-constituent and the plant extracts with pooled CYP450 were significantly lesser (p<0.05; p<0.01; P<0.001) than known CYP450 inhibitors. The present findings suggested that plant extract and its bioactive compound contributed negligible herb drug interactions and safe to use in diabetes management.