Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/33413| Title: | Detection of white spot syndrome virus (WSSV) in the pacific white shrimp Litopenaeus vannamei in southern India using PCR, SEM and histological techniques |
| Authors: | Haq, M A Badhul Durgadevi, K Banu, M Nirosh Ahamad, A Sajith Tiwary, Chandan Srinivasan, M |
| Keywords: | Litopenaeus vannamei;PCR;Penaeus monodon;SEM;White spot syndrome virus (WSSV) |
| Issue Date: | Jul-2015 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Outbreak of disease is one of the major stumbling blocks in the development and sustainability of aquaculture. White spot syndrome virus (WSSV), the causative virus of the white spot disease, was found in most shrimp farming areas of the world, where it causes large economic losses to the shrimp farming industry. The objective of the present investigation was to determine the level of WSSV transmission from the infected tiger prawn Penaeus monodon to specific pathogen free (SPF) Litopenaeus vannamei in WSSV impact region of Tamil Nadu. In our study, we performed polymerase chain reaction (PCR), histological observation and scanning electron microscopy to detect WSSV. Our nested PCR revealed the bands ranging from 296 to 910 bps in WSSV infected L. vannamei shrimps. The infected shrimp showed 20 to 2000 copies of viral DNA. Histological surveillance was performed on infected L. vannamei at cephalothorax region. We observed intranuclear inclusion bodies characteristic in the gill region of infected L.vannamei. No significant difference was noticed in the hematopoietic tubules of WSSV affected L.vannamei. However, in the higher magnification, more than 15 kV × 150 the hematopoietic tubules were found at 10 μm in diameter. Basically, the study was initiated to document the primary instance of WSSV infected shrimp, which was new imported species. Overall, the investigation provides information on the level of pathogenicity of WSSV in different species of L. vannamei including SPF and native species. |
| Page(s): | 369-375 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.14(3) [July 2015] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 14(3) 369-375.pdf | 421.17 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.