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http://nopr.niscpr.res.in/handle/123456789/33414Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Gauthami, S | - |
| dc.contributor.author | Meena, Keerti | - |
| dc.contributor.author | Kumar, Deepak | - |
| dc.contributor.author | Uma, Madala | - |
| dc.contributor.author | Reddy, Y Vishnuvardhan | - |
| dc.contributor.author | Rao, Pavuluri Panduranga | - |
| dc.contributor.author | Hegde, Nagendra R | - |
| dc.date.accessioned | 2015-11-23T10:31:28Z | - |
| dc.date.available | 2015-11-23T10:31:28Z | - |
| dc.date.issued | 2015-07 | - |
| dc.identifier.issn | 0975-0967 (Online); 0972-5849 (Print) | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/33414 | - |
| dc.description | 376-381 | en_US |
| dc.description.abstract | A method to prepare a concentrated monophasic phenol guanidinium isothiocyanate-based reagent for extraction of RNA from liquid samples is described. The reagent was comparable to a commercial reagent for extraction of genetic material of several RNA viruses as assessed by limit of detection of RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The study showed that the concentrated monophasic reagent could be used in place of a commercial reagent. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | NISCAIR-CSIR, India | en_US |
| dc.rights |  CC Attribution-Noncommercial-No Derivative Works 2.5 India | en_US |
| dc.source | IJBT Vol.14(3) [July 2015] | en_US |
| dc.subject | Acid phenol | en_US |
| dc.subject | RNA isolation | en_US |
| dc.subject | RT-PCR | en_US |
| dc.subject | Virus R NA | en_US |
| dc.title | MPGIT: A concentrated monophasic phenol-guanidinium isothiocyanate reagent for isolation of viral RNA | en_US |
| dc.type | Article | en_US |
| Appears in Collections: | IJBT Vol.14(3) [July 2015] | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 14(3) 376-381.pdf | 165.68 kB | Adobe PDF | View/Open |
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