<span style="mso-bidi-language: KOK" lang="EN-US">The effect of agitation and aeration on production of cyclosporin A <span style="mso-bidi-language:KOK" lang="EN-US">in batch cultures of <i>Tolypocladium inflatum </i><span style="mso-bidi-font-style:italic" lang="EN-US">MTCC 557 </span></span></span>

Abstract

<span style="mso-bidi-language:KOK" lang="EN-GB">The present study reports the effects of aeration and agitation on growth and production of the cyclosporin A (CyA)<span style="mso-bidi-language:KOK" lang="EN-GB"> in batch fermentor cultures of <i>Tolypocladium inflatum </i>MTCC 557. Dissolved oxygen concentration, pH, dry cell wt, CyA titer and sugar utilization were continuously measured and determined. In the first step, <span style="mso-bidi-language:KOK" lang="EN-GB">the effect of agitation was evaluated by<b> </b>changing the agitation speed in the range of 250 to 450 rpm at 1 vvm. Further, after selecting the optimum agitation speed, the aeration rate was varied between 0.5 vvm to 1.0 vvm. Efficiency of aeration and agitation was evaluated through oxygen mass transfer coefficient (<i>k</i>_L<i>a</i>). Maximum CyA production of 1274 mg/L with specific productivity of 0.152 mg/g.h was obtained at 350 rpm agitation and 0.75 vvm aeration.<span style="mso-bidi-language:MR" lang="EN-GB"> The <i>k</i>_L<i>a </i>of the fermentation system supporting maximum production (350 rpm, 0.75 vvm) was 0.113 h^-1. The oxygen transfer rate (OTR), oxygen utilization rate (OUR), and specific oxygen uptake rates (qO₂) in the fermentation broth were also determined.

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