Molecular characterization of Assam Local pig

Abstract

The detailed genetic diversity of Assam Local pig population was studied through microsatellite markers. Most of the studied loci were polymorphic in nature and a total of 163 alleles were observed across all the loci. The range of alleles was found to be from 2 to 11 with a mean of 6.52±2.311. The frequency distribution of microsatellite alleles in the population was from 0.0208 to 0.9091. The overall mean of observed and expected heterozygosities were 0.50±0.272 and 0.71±0.182, respectively. The polymorphism information content (PIC) was 0.67±0.184 and Shannon’s information index (I) was sufficiently high with a mean of 1.53. The within population inbreeding estimate (F_IS) value (0.273) indicated shortfall of heterozygosity in the population. The bottleneck analysis revealed that population had not undergone any recent reduction.