Statistical Optimization for Enhanced Production of Cellulase from Sugarcane Bagasse using Response Surface Methodology

Abstract

The study describes the isolation and identification of fungus <i style="mso-bidi-font-style: normal">Trichoderma longibrachiatum</i> (GENBANK accession number: KM274866) and the optimum solid state fermentation conditions required for cellulase production based on Response Surface Methodology (RSM) using Taguchi model. When different lignocellulosic substrates were used, sugarcane bagasse supported highest (0.169 U/ml) cellulase production. Statistical optimization using Taguchii model revealed culture parameters such as Lactose which was a carbon source and cellulase inducer, CaCl₂ and temperature to significantly influence cellulase production. These parameters were further optimized using RSM, and the optimal concentrations for growth of the organism was found to be 0.382 mg/ml for lactose and 1.932 mgs/ml for CaCl₂ at an optimal temperature of 30°C. Under optimal media conditions an 8 fold increase in cellulase production was observed.