Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/33895| Title: | Isolation and HPLC profiling of chemical constituents of Saraca asoca stem bark |
| Authors: | Ahmad, Furkan Misra, Laxminarain Tewari, Rashi Gupta, Preeti Gupta, Vivek K Darokar, Mahendra P |
| Keywords: | Saraca asoca (Roxb.) de Wilde. syn. S. indica Linn.;Antibacterial activity;HPLC profiling |
| Issue Date: | Mar-2016 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Saraca
asoca (Roxb.)
de Wilde, a common tree of India, is popularly used in the ayurvedic and modern
herbal systems of medicine for genito-urinary problems of women. In the current
herbal market there happens to be mixing of adulterants with authentic S. asoca stem bark. In order to identify
the right bark material, there exists hardly any laboratory testing
possibilities to authenticate the stem bark samples. The n-hexane, methanol and aqueous methanol extracts have been
investigated by isolating and characterizing major compounds and developing the
HPLC profiling of methanol and aqueous methanol extracts. The n-hexane, methanol and 60% aqueous
methanol extracts of stem bark of S.
asoca after isolation, have yielded a total of 17 compounds whose
structures were established by spectroscopic methods. A series of experiments
were run to develop the HPLC conditions for the chemical profiling of the bark.
The n-hexane extract has been found
to contain mainly triterpenoids and sterols while methanol extract has yielded
several flavanol derivatives along with a few triterpenoids. The aqueous
methanol extract afforded flavanol derivatives. Out of the isolated compounds,
ursolic acid, lupeol, glochidiol, 5,3'-dimethoxy epicatechin,
3'-eoxyepicatechin-3-O-β- -glucopyranoside,
3'-deoxycatechin-3-O-α- -rhamnopyranoside
and epigallocatechin have been isolated from S. asoca bark for the first time. HPLC profiling of methanol and
aqueous methanol extracts of the bark has been developed by using mainly the
isolated compounds as references. The percent composition of the major
compounds was determined by HPLC and can be used to monitor the possible
adulterations in the commercial bark materials. The antibacterial tests showed
that the methanol and aqueous methanol extracts and few compounds have mild
activity on some of the bacterial strains whereas they did not show any
antifungal activity. |
| Page(s): | 353-361 |
| ISSN: | 0975-0983(Online); 0376-4699(Print) |
| Appears in Collections: | IJC-B Vol.55B(03) [March 2016] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJCB 55B(3) 353-361.pdf | 222.73 kB | Adobe PDF | View/Open |
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