<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Influence of root-knot nematode infestation on antioxidant enzymes, chlorophyll content and growth in <i style="mso-bidi-font-style:normal">Pogostemon cablin</i> (Blanco) Benth.</span>

Abstract

<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">Plants adapt themselves to overcome adverse environmental conditions, and this involves a plethora of concurrent cellular activities. Physiological experiments or metabolic profiling can quantify this response. Among several diseases of <i style="mso-bidi-font-style:normal">Pogostemon cablin</i> (Blanco) Benth. (Patchouli), root-knot nematode infection caused by <i style="mso-bidi-font-style: normal">Meloidogyne incognita</i> (Kofoid and White) Chitwood causes severe damage to the plant and hence, the oil production. In the present study, we identified <i style="mso-bidi-font-style:normal">M. incognita</i> morphologically and at molecular level using sequenced characterized amplified region marker (SCAR). <i style="mso-bidi-font-style:normal">M. incognita</i> was artificially inoculated at different levels of second stage juveniles (J₂) to examine the effect on Patchouli plant growth parameters. Peroxidase and polyphenol oxidase enzyme activity and changes in the total phenol and chlorophyll contents in <i style="mso-bidi-font-style:normal">M. incognita </i>was also evaluated in response to infection. The results have demonstrated that nematode infestation leads to increased peroxidase activities in the leaves of the patchouli plants and thereby, increase in phenolic content as a means of defence against nematode infestation. Chlorophyll content was also found decreased but no changes in polyphenol oxidase enzyme activity.</span>