<span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Improvement of banana cv. Rasthali (Silk, AAB) against Fusarium oxysporum f.sp. cubense (VCG 0124/5) through induced mutagenesis: Determination of LD50 specific to mutagen, explants, toxins and in vitro and in vivo screening for Fusarium wilt resistance

Saraswathi, MS; Kannan, G; Uma, S; Thangavelu, R; Backiyarani, S

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DC Field	Value	Language
dc.contributor.author	Saraswathi, MS	-
dc.contributor.author	Kannan, G	-
dc.contributor.author	Uma, S	-
dc.contributor.author	Thangavelu, R	-
dc.contributor.author	Backiyarani, S	-
dc.date.accessioned	2016-05-01T16:08:15Z	-
dc.date.available	2016-05-01T16:08:15Z	-
dc.date.issued	2016-05	-
dc.identifier.issn	0975-1009 (Online); 0019-5189 (Print)	-
dc.identifier.uri	http://hdl.handle.net/123456789/34158	-
dc.description	345-353	en_US
dc.description.abstract	Shoot tips and in vitro grown proliferating buds of banana cv. Rasthali (Silk, AAB) were treated with various concentrations and durations of chemical mutagens viz., EMS, NaN₃and DES. LD₅₀ for shoot tips based on 50% reduction in fresh weight was determined as 2% for 3 h, 0.02% for 5 h and 0.15%for 5 h, while for proliferating buds, they were 0.6% for 30 min, 0.01% for 2 h and 0.06% for 2 h for the mutagens EMS, NaN₃ and DES, respectively. Subsequently, the mutated explants were screened in vitro against fusarium wilt using selection agents like fusaric acid and culture filtrate. LD₅₀ for in vitro selection agents calculated based on 50% survival of explants was 0.050 mM and 7% for fusaric acid and culture filtrate, respectively and beyond which a rapid decline in growth was observed. This was followed by pot screening which led to the identification of three putative resistant mutants with an internal disease score of 1 (corm completely clean, no vascular discolouration). The putative mutants identified in the present study have also been mass multiplied in vitro.	en_US
dc.language.iso	en_US	en_US
dc.publisher	NISCAIR-CSIR, India	en_US
dc.rights	CC Attribution-Noncommercial-No Derivative Works 2.5 India	en_US
dc.source	IJEB Vol.54(05) [May 2016]	en_US
dc.subject	Di ethyl sulphate	en_US
dc.subject	Ethyl methane sulfonate	en_US
dc.subject	Fusaric acid	en_US
dc.subject	Fungal resistance	en_US
dc.subject	Musa sp.	en_US
dc.subject	Sodium azide	en_US
dc.title	Improvement of banana cv. Rasthali (Silk, AAB) against Fusarium oxysporum f.sp. cubense (VCG 0124/5) through induced mutagenesis: Determination of LD₅₀ specific to mutagen, explants, toxins and in vitro and in vivo screening for Fusarium wilt resistance	en_US
dc.type	Article	en_US
Appears in Collections:	IJEB Vol.54(05) [May 2016]

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