<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman";mso-ansi-language:EN-US;mso-fareast-language: EN-US;mso-bidi-language:AR-SA" lang="EN-US">Influence of temperature on symptom expression, detection of host factors in virus infected <i style="mso-bidi-font-style:normal">Piper nigrum </i>L.</span>

Abstract

<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:"times="" roman";="" mso-bidi-font-family:"times="" roman";letter-spacing:.1pt;mso-ansi-language:="" en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">Expression of symptoms in black pepper plants<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:"times="" letter-spacing:.1pt;mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US"> </span></i><span style="font-size:11.0pt; mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-bidi-font-family:"times="" roman";letter-spacing:.1pt;="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">(<i style="mso-bidi-font-style:normal">Piper nigrum</i>)<span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:"times="" letter-spacing:.1pt;mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US"> infected with Piper yellow mottle virus (<span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:"times="" letter-spacing:.1pt;mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US">PYMoV) <span style="font-size:11.0pt; mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";mso-fareast-font-family:="" "times="" roman";mso-bidi-font-family:"times="" roman";letter-spacing:.1pt;="" mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa"="" lang="EN-US">vary depending on the season, being high during summer months. Here, we explored the influence of temperature on symptom expression in <span style="font-size:11.0pt;mso-bidi-font-size:9.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:"times="" letter-spacing:.1pt;mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US">PYMoV infected <i style="mso-bidi-font-style:normal">P</i>.<i style="mso-bidi-font-style:normal"> nigrum</i><span style="font-size:11.0pt;mso-bidi-font-size:10.0pt;font-family:" times="" new="" roman";="" mso-fareast-font-family:"times="" roman";mso-bidi-font-family:"times="" letter-spacing:.1pt;mso-ansi-language:en-us;mso-fareast-language:en-us;="" mso-bidi-language:ar-sa"="" lang="EN-US">. Our controlled environment study revealed increase in virus titer, total proteins, IAA and reducing sugars when exposed to temperature stress. There was change in the 2-D separated protein before and after exposure. The 2-D proteomics LC-MS identified host and viral proteins suggesting virus-host interaction during symptom expression. The analysis as well as detection of host biochemical compounds may help in understanding the detailed mechanisms underlying the viral replication and damage to the crop, and thereby plan management strategies.</span></span></span></span></span></span></span>