<i style="mso-bidi-font-style:normal"><span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB">Dillenia indica</span></i><span style="font-size:11.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB; mso-fareast-language:EN-US;mso-bidi-language:HI" lang="EN-GB"> L. exhibited hematoprotective effects via modulation of antioxidant responsive enzymes</span>

Abstract

<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">The present studies were intended towards finding a suitable plant based natural product which can ameliorate the hematotoxicity encountered as<b style="mso-bidi-font-weight:normal"> </b>adverse drug reactions (ADRs) in various therapeutic regimens.<b style="mso-bidi-font-weight:normal"> </b><i style="mso-bidi-font-style:normal">Dillenia indica</i> (DI) is used in folklore and traditional medicine as general health enhancer. The aim of the study was to evaluate the hematoprotective effects of natural botanical, <i style="mso-bidi-font-style:normal">Dillenia indica </i>(DI) ethanolic extract (DIEE), employing a Phenylhydrazine (Phz)-induced hematotoxicity model of Sprague Dawley (SD) rats. DIEE exhibited hematoprotective effects <i style="mso-bidi-font-style:normal">In vitro</i> and <i style="mso-bidi-font-style: normal">In vivo</i>. Solvent guided Fractionation studies revealed the presence of high amounts triterpenoids in DIEE. DIEE showed radical scavenging properties in DPPH radical scavenging assay. Mechanism based studies unveiled the positive effects of DIEE on antioxidant enzymes Superoxide dismutases 2 and 3 (SOD’s) and Nuclear erythroid 2 p45-related factor 1 (Nfer- 1) and Nuclear erythroid 2 p45-related factor 2 (Nfer-2) employing quantitative real time PCR. In toxicity studies in SD rats, DIEE imparted no toxicity. The results are of integral significance as, the study establishes the folklore and traditional medicine claims about the peremptory actions of DI.</span>