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Title: Multigene DNA barcodes of Indian strain Labidocera acuta (Calanoida: Copepoda)
Authors: Jagadeesan, L
Perumal, P
Francis, K X
Biju, A
Keywords: mtCOI;DNA barcoding;ITS-2;Labiodcera acuta;18S rRNA
Issue Date: Jan-2016
Publisher: NISCAIR-CSIR, India
Abstract: The present study was performed to establish the multigene DNA barcodes of Labidocera acuta (collected from Parangipettai, India) and to describe their genetic divergence and phylogenetic relatedness with other related species. Three different gene markers, viz., mitochondrial cytochrome oxidase I (mtCOI), 18S ribosomal RNA (18S rRNA) and internal transcribed spacer-2 (ITS-2), were used for the study. Each marker showed different kinds of genetic divergence and relatedness within and between the species. The mtCOI and ITS-2 gene sequences of L. acuta showed less genetic divergences (0.9%, mtCOI & 2.2%, ITS-2) to conspecific individuals, however it showed considerable sequence differences with the intrageneric (18.5%, mtCOI & 36.2%, in ITS-2) and intergeneric (17.2-21.8%, mtCOI & 30.8-37.6 %, ITS-2) species. 18S rRNA marker sequences showed considerable sequence difference between the intergeneric (4.5-6.9 %) individuals, whereas it showed less genetic divergence with the intrageneric individuals. The DNA sequences of L. acuta provide definite differentiation between the closely related species even if they had more resemblance in their morphology.
Page(s): 57-63
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.15(1) [January 2016]

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