<i><span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">In vitro</span></i><span style="font-size:11.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB"> micropropagation of <i>Simarouba glauca </i>DC.</span>

Abstract

<span style="font-size:11.0pt;font-family: " times="" new="" roman";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">An efficient and improved micropropagation protocol was developed from different explants of 2-month-old seedlings of <i style="mso-bidi-font-style:normal">Simarouba glauca</i><span style="mso-bidi-font-style:italic"> DC<i style="mso-bidi-font-style:normal">. </i>The highest number of shoots (55.3) with 100% response was obtained from cotyledonary-node explant on MS medium fortified with 6-benzyl adenine (BA; 3.0 mg/L), kinetin (KN; 0.5 mg/L), α-naphthalene acetic acid (NAA; 0.5 mg/L) and glutamine (10 mg/L). Further, the highest rooting response (95%) with 15 roots of 5.8 cm root length was observed on MS medium supplemented with NAA (3.0 mg/L). The rooted plantlets were hardened by transferring to paper containers with soil and sand (2:1) and kept in shade for acclimatization. These plants were further transferred to the field with a survival percentage of 81.5%.</span></span>