Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/34591| Title: | Screening, phenotypic and genotypic identification of β-carotene producing strains of Dunaliella salina from Thailand |
| Authors: | Sathasivam, Ramaraj Praiboon, Jantana Chirapart, Anong Trakulnaleamsai, Savitr Kermanee, Prasart Roytrakul, Sittiruk Juntawong, Niran |
| Keywords: | Screening;Morphology;RLFP;18S rDNA;ITS;rbcL gene;β-carotene;Dunaliella salina |
| Issue Date: | Dec-2014 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Dunaliella salina is a salt-loving microalga that accumulates high amounts of β-carotene when cultivated under unfavorable conditions. In this study we aimed to screen β-carotene producing strains of D.salina from salt soil samples collected from 19 provinces in the northeastern part of Thailand. For preliminary screening 70 pure isolated strains were screened by using 18S rDNA conserved primers (MA1&MA2), only 15 isolates produced a band (~2100 bp) as that of D.salina. For the observation of β-carotene production, these 15 isolates were grown on agar plate containing modified Ramaraj medium with 1.0 M NaCl under continuous illumination. After a month only 3 isolates completely turned red. However, by using the banding pattern produced by 18S rDNA primers, the 15 isolates could not be differentiated between the β-carotene and non β-carotene producing strains. Comparing the above results we found that the observation of β-carotene production on agar plate is more easy and suitable method than molecular technique to screen β-carotene producing strains. Morphological characteristics of the 3 screened Thai isolates clearly delineated that it belongs to the genus Dunaliella. ITS-RFLP banding pattern, 18S rDNA, ITS and RuBisCo large subunit (rbcL) sequences were used to confirm at the species level. These results indicated that these 3 isolates are D.salina and were named D.salina strain KU07, D.salina strain KU11 and D.salina strain KU13. Under non-stress conditions these 3 strains had an ability to accumulate β-carotene up to 5.61±0.25, 7.58±0.19 and 6.73±0.32 pg/cell, respectively. |
| Page(s): | 2198-2216 |
| ISSN: | 0975-1033 (Online); 0379-5136 (Print) |
| Appears in Collections: | IJMS Vol.43(12) [December 2014] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJMS 43(12) 2198-2216.pdf | 1.04 MB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.