<span style="font-size:15.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Identification of new <i style="mso-bidi-font-style:normal">Streptomyces griseus</i> strains with potential antimicrobial activity isolated from Caspian Sea</span>

Abstract

<span style="font-size:9.0pt;font-family: " times="" new="" roman","serif";mso-fareast-font-family:"times="" roman";mso-bidi-font-family:="" mangal;mso-ansi-language:en-gb;mso-fareast-language:en-us;mso-bidi-language:="" hi"="" lang="EN-GB">With the emergence of new antibiotic-resistant bacteria, the global announcement for new antimicrobial agents has initiated. Many scientists have been focused on identification and characterization of novel marine species which produce more potent antimicrobial agents. In this study, 162 strains were isolated from Caspian Sea and analyzed based on antimicrobial activity. Bacteria were grown in wide period of time, 24 h to 20 days, on Muller Hinton Agar medium. Among 162 isolates, 4 strains showed antimicrobial activity to <i>Bacillus subtilis</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> as reference strains. But the maximum effect was observed on <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i> (Gram positive bacteria). The positive strains were subjected to 16S rDNA PCR sequencing and the results were BLASTed against the NCBI database to evaluate genetic distribution and bacterial classification. The strains were submitted to NCBI as new Streptomyces griseus strains.</span>