The best DNA extraction method from sea anemone species with the mucosal tissue

Abstract

DNA extraction is the first and the most important step in genomic analysis of desoxy ribonucleique acid (DNA) for subsequent activities. DNA isolation is very difficult from sea anemones due to the mucosal contaminants. Different protocols are suggested for DNA extraction from sea anemones. The present study provides a DNA extraction protocol by some changing in common Phenol Chloroform method. This method was modified during the examination to achieve the best result. Sea anemones were collected from the southeast coasts of Iran, Oman Sea. They were transported to the laboratory and kept alive under in vitro conditions. The DNA was extracted by Phenol Chloroform/Isoamyl alcohol (PCI) method and extracted DNA was used as template for amplification in PCR. We proposed a modified PCI method in this study.