Total phenolic content and in-vitro antioxidant activities from methanolic extract of alligator pipefish, Syngnathoides biaculeatus (Bloch, 1785)

Abstract

Alligator pipefish, Syngnathoides biaculeatus (Bloch, 1785) is one of the heavily traded and expensive ingredient in traditional Chinese medicines and there were no reports on its antioxidant activities. Total phenolic content (TPC) and in-vitro antioxidant activities in terms of reducing power (RP), metal chelating activity (MCA), 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), inhibition of lipid peroxidation (LPX) and hydroxyl radical scavenging (HRS) activity were evaluated at extract concentrations ranging between 200 and 800 µg. Levels of TPC, RP, MCA, DPPH scavenging, FRAP, LPX and HRS activity measured was 19.81±1.10 to 64.04±0.45 mg gallic acid g-1 dried extract, from 0.16 to 0.31 Abs, from 6.20±0.21% to 29.33±1.73%, from 16.59±0.01 to 40.21±0.02%, from 75.46±0.90 to 167.25±0.41 mg ascorbic acid g-1 dried extract, from 54.59±1.5% to 84.28±3.5% and from 84.89±0.26 to 91.02±0.01%, respectively. Significant correlation (P<0.05) between TPC and other antioxidant properties confirm the antioxidant potential of S. biaculeatus. Results of this study would form a baseline information on the antioxidant potential of alligator pipefish.