Biodegradation and Detoxification of a Textile Azo Dye Reactive Blue 171 by Marinobacter sp. NB-8

Abstract

The degradation and detoxification of an azo dye Reactive Blue 171 was carried out by the acclimatized Marinobacter sp. NB-8 (Accession No. HF541878) isolated from marine environment and identified by 16s rRNA sequence analysis. Decolorization of the dye was determined by spectroscopic technique. Isolated Marinobacter sp.NB-8 could decolorize the 1500µg/ml dye up to 92.00% in the nutrient medium within 24 hours. Slight decrease in decolorization was observed when half strength nutrient medium was used. Decolorization of the dye was studied in presence of different co-substrates Viz. 1% glucose, 1% yeast extract and 1% starch and found that percent decolorization was up to 92.22%, 94.00% and 92.88% respectively. Percent decolorization by cell free extract was also studied. Isolate could reduce the COD of the dye Reactive Blue 171 up to 80.78% after 24 hours. Degradation of the dye was confirmed by GC-MS analysis.