Antifouling Potentials of Mangrove Rhizophora apiculata against Vibrio alginolyticus strainVBM-01

Abstract

Present study consist the antifouling properties from Rhizophora apiculata was tested against pioneering marine biofouling bacteria Vibrio alginolyticus. The Vibrio alginolyticus was found to dominant group with high population density of 160±2.0×10-5 colony forming unit which has been isolated from Mandapam coast of South India. Agar well diffusion method was used to find out the inhibition of biofilm forming bacteria through zone of inhibition. Followed by the time kill assay and anti-adhesion assay was performed by exploiting the potentials of antibiofilm activity. Preliminary phyto-chemical screening shows the alkaloids and saponins were exist in the chloroform extract of Rhizophora apiculata. Further, HPLC chromatogram shows two significant major peaks with 27.943 and 98.670 retention time. HPTLC profile was recorded with Rf value concluded the broad antifouling properties of the chloroform extracts of Rhizophora apiculata inhibit the growth of pioneering marine biofouling bacteria.