Patterning of Vibrio spp. on oxide dots of silicon for the development of biosensor

Abstract

Block Spot Syndrome is one of the most serious bacterial diseases in Penaeus monodon (Fab.) caused by Vibrio spp. which mainly affects the fresh water prawn industry and marine ecosystem. In this study, a unique attempt is made to determine this bacterial infection by attaching the V. harveyi cells on pre-determined oxide dots on silicon substrate. Silicon oxide dots fabricated by using an in-house made device, is comparably inexpensive, trouble-free and fast for large area patternings than the Atomic Force Microscopy (AFM) anodic oxidation method. The attachment of Vibrio spp. on the silicon oxide pattern is confirmed by the Scanning Electron Microscopy (SEM) surface morphology and topography analysis by Atomic Force Microscopy (AFM).