Optimization of microbial hydrogen production using statistical experimental design from maize stalk by isolated strain

Abstract

Experimental designs have been applied for optimizing the process parameters for hydrogen production from maize stalk hydrolyzate by a newly isolated facultative strain. Using Plackett-Burman design glucose, yeast extract, malt extract, peptone, and NaCl have been identified as significant variables influencing hydrogen production and these variables are subsequently optimized using a central composite design (CCD). Box-Behnken design has been adopted for optimizing the process parameters for maximum hydrogen production. Using this statistical optimization method a maximum hydrogen yield of 0.91 mol H₂/mol glucose has been achieved.