Diversity analysis of wheat cultivars on the basis of solubility-fractionated seed storage protein polymorphism

Abstract

Wheat seed storage proteins play important role in determining its quality. At the same time, polymorphism of wheat seed storage proteins can be explored to analyze genetic diversity among wheat cultivars. In the present study, a simple process of solubility-based sequential fractionation of wheat seed storage proteins has been documented. Using the same, 16 polymorphic protein bands, belonging to the class albumin, globulin, gliadin and glutenin, have been recorded in 10 wheat cultivars. On the basis of the recorded polymorphism, all the wheat cultivars could be distinguished from each other. Of the 10 cultivars, the cultivar DBW 39 was found to have the unique composition of seed storage proteins. This information can now be further explored for varietal screening and identification in wheat breeding programme aiming towards quality improvement in wheat.