Molecular evidence of Chili vein mottle virus and Chilli leaf curl virus simultaneously from naturally infected chilli plants (<i>Capsicum</i> <i>annuum</i> L.)

Abstract

The occurrence of <i>Chilli leaf curl virus</i> (ChLCV) and <i>Chilli vein mottle virus</i> (CVMV) were detected by using the duplex PCR in the mixed infected Chilli plants (<i>Capsicum annuum</i> L.). The duplex PCR was done by using the specific primer Pot 1 and Pot 2 for CVMV and AVF28 and AV29R for ChLCV. The amplicon and the sequence analysis confirmed the presence of potyvirus and begomovirus in the mixed infection. This combinations exhibited synergistic symptoms and large numbers of cells were doubly infected. This paper reports highly specific conventional PCR assays for detection of two independent viruses infecting chilli plants simultaneously.