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http://nopr.niscpr.res.in/handle/123456789/3802| Title: | Purification and partial characterization of cytochrome c552 from Halobacterium salinarium |
| Authors: | Sreeramulu, K |
| Keywords: | Cytochrome c552;Halobacterium salinarium;purification;characterization |
| Issue Date: | Aug-2003 |
| Publisher: | CSIR |
| Abstract: | Cytochrome c552 was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be 14.1 kD. |
| Page(s): | 274-277 |
| ISSN: | 0301-1208 |
| Appears in Collections: | IJBB Vol.40(4) [August 2003] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBB 40(4) 274-277.pdf | 106.05 kB | Adobe PDF | View/Open |
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