Identification of mixed infection caused by Badnavirus and CMV in Jasmine (<em>Jasminum multiflorum</em> Roth)

Abstract

The symptoms of chlorotic ring spots and irregular chlorotic patches were recently observed on leaves of landscape-grown jasmine (<em>Jasminum multiflorum</em> Roth) in southern parts of India. The causal agent was mechanically transmitted from symptomatic leaves of jasmine to <em>Nicotiana glutinosa</em>, <em>Capsicum annum</em>, <em>Solanum lycopersicum</em>, <em>Cucurbita pepo</em> and <em>Cucumis sativus</em>. Leaf dip preparations from virus-infected plants in electron microscopy revealed the presence of isometric particles similar to <em>Cucumber mosaic virus </em>(CMV) and bacilliform virus particles of badnavirus. The virus reacted specifically with IgG for CMV and Banana streak virus (BSV) in direct antigen coating, enzyme-linked immunosorbent assay. No reaction was observed with ilar-, poty- and tospo-viruses specific IgG. Reverse transcription polymerase chain reaction with total RNA isolated from symptomatic jasmine leaves and infected <em>N. tabacum </em>'Xanthi' using CMV coat protein (CMV CP) specific primers amplified the expected product. The association of badna virus with <em>jasmine</em> was confirmed by PCR amplification, cloning and sequencing of 560 bp amplicon corresponding to the reverse transcriptase and ribonuclease H coding region in open reading frame III. The sequence analysis revealed maximum identity to badna virus group Diascorea bacilliform virus (88.5% at nt level) and CMV group IB (96% at nt level). To our knowledge, this is the first report of CMV and badna mixed infection in jasmine in India.