Quantitative aspects of intramolecular interactions in ternary complexes of purine nucleotides and amino acids

Abstract

Interaction Of bivalent metal ions (CO²⁺,Ni²⁺, Cu²⁺,Zn²⁺) with purine nucleotides (5' - guanosinemono-phosphate and 5' - inosinemonophonsphate) and amino acids (alanine, phenylalanine and tryptophan) in I: I and I: I: 1 ratios has been investigated by potentiometric <em>p</em>H measurements and formation constants in various systems are determined by computer program. The extra stabilization is expressed in terms of ∆log<em>K</em>. The hydrophobic interactions associated with different amino acids and nucleotides have been identified with parameters like ∆log<em>K</em>, K_l % of (MLA)_st' and ∆<em>G</em>^o The pH profiles of various species are generated in order to identify the stable species.