Cultivation of <i>Spirulina platensis </i> in different selective media

Abstract

Experiments were carried out to assess the optimum culture conditions for the growth of <i>S.platensis</i> in different medium <i>viz</i>, Zarrouk medium, BG11 medium, Conway medium, F/2 medium and Sea water. Growth analyses and dry weight were monitored for 30 days on daily basis. pH was found to be ranged from 9.1 to 11.0 in Zarrouk medium, 8.9 to 9.2 Conway in F/2 medium and 8.57 to 8.68 Seawater medium. Room temperature was maintained at 30 ± 2 °C under 12/12 hour light-dark cycles, light illuminated (4500 lux) and Spectrophotometer used at 550nm, 680nm for two days interval. Dry weight (DW) was gradually increased along with the age of culture and 1.86 dw/L was achieved in Zarrouk medium. The <i>S.platensis</i> inoculated in Conway and F/2 medium were survived but well the growth was not flourished, achieved the maximum dry weight of 0.52dw/L on 21^th day of cultivation. Seawater fortified with different amount of NaHCO3 and NaNO3 did not show any significant impact on <i>Spirulina </i> growth. However, results of the present investigation could be considered for commercial cultivation of <i>Spirulina </i> using Natural medium.