Isolation and cellular fatty acid profile analyzation of two marine bioluminescent bacteria

Abstract

Two luminescent bacterial strains KOOS1 and KOOS2 isolated from surface mucus of <i>Octopus </i> sp. collected from Andaman were identified by their cellular fatty acid composition analyzation with the help of Microbial Identification system (MIDI). SIM indexes obtained for these isolated strains were 0.772 (KOOS1) and 0.754 (KOOS2) respectively and were identified as <i>Photobacterium damselae</i> and <i>Vibrio fischeri</i>. Major fatty acids found in <i>Photobacterium damselae </i> were Saturated: Dodecanoic acid (C_12:0), Tetradecanoic acid (C_14:0), Pentadecanoic acid (C_15:0), Hexadecanoic acid (C_16:0), Heptadecanoic acid (C_17:0) and Octadecanoic acid (C_18:0); and Unsaturated: 3-hydroxy-9-methyl decanoic acid (C_11:0iso 3OH), 3-hydroxydodecanoic(C_12:0 3OH), C_16:1ω5c, Oleic acid (C_18:1ω9c) and C_18:1ω5c.In <i>Vibrio fischeri </i> Saturated: C_12:0, Tridecanoic acid (C_13:0), C_15:0, C_16:0, C_17:0 and C_18:0; and Unsaturated: C_11:0iso 3OH,2-hydroxydodecanoic (C_12:0 2OH), C_12:03OH, C_13:0iso, C_14:0iso, C_15:0iso, C_15:0anteiso, C_16:0iso, C_17:0iso, C_16:1ω5c, C_15:0iso3OH, C_17:1 ω8c and C_17:1ω6c were found. Cyclopropane acids have not been detected in both <i>Photobacterium damselae </i> and <i>Vibrio fischeri</i>.